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Microsatellite-assisted backcross selection in maize Genet. Mol. Biol.
Benchimol,Luciana Lasry; Souza Jr.,Cláudio Lopes de; Souza,Anete Pereira de.
A microsatellite marker (SSR) was chosen to simulate a target allele and three criteria (02, 04 and 06 markers per chromosome) were tested to evaluate the most efficient parameters for performing marker-assisted backcross (MAB) selection. We used 53 polymorphic SSRs to genotype 186 BC1 maize (Zea mays L.) plants produced by crossing the inbred maize lines L-08-05 (donor parent) and L-14-4B (recurrent parent). The second backcross (BC2) generation was produced with 180 plants and screened with markers which were not recovered from the first backcross (BC1) generation. A total of 480 plants were evaluated in the third backcross (BC3) generation from which 48 plants were selected for parental genotype recovery. Recurrent genotype recovery averages in three...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maize; Marker-assisted backcrossing (MAB); Marker-assisted selection; Microsatellites.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500022
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